European Companies Search Engine
EU funding (€245,732): Dynamic regulation of paracellular channel gating Hor7 Apr 2020 EU Research and Innovation programme "Horizon"
Text
Dynamic regulation of paracellular channel gating
Epithelial paracellular, i.e., tight junction, permeability is largely defined by the integrated functions of claudin proteins that can either seal the paracellular space or form highly-selective conductance channels. The importance of claudins is exemplified by the many human diseases caused by barrier dysregulation and claudin mutations. The host laboratory recently reported the first measurements of single channel tight junction currents, thereby demonstrating that claudin channels transition between open and closed states. The central hypothesis of this application is that claudin channel activity is regulated by specific molecular interactions. Unfortunately, the trans-tight junction patch-clamp method developed by the host laboratory is extremely labor intensive and unable to capture more than a small section of a single tight junction, making it unsuitable for comprehensive analyses. To overcome this obstacle, we first aim to develop a nanopillar array chip that will supersede the patch-clamp method. Cells grown over and around the nanopillars will form tight junctions above the nanoelectrode at the tip of each nanopillar. This will make it possible to measure large numbers of single-channel events over many junctions. The second aim will exploit the nanopillar chip to define the conductances and gating activities of claudin proteins and the mechanisms by which they are regulated. This novel technology will also allow others to analyze claudin function in health and disease. The nanopillar chip and data generated using this tool will accelerate our understanding of tight junction biology and enable development of channel modulators that, in a manner analogous to the advances enabled by transmembrane ion channel modulators, will lead to novel therapeutic approaches.
Funded Companies:
| Company name | Funding amount |
| ? | |
| Agencia Estatal Consejo Superior de Investigaciones Cientificas | €245,732 |
Source: https://cordis.europa.eu/project/id/896293
The filing refers to a past date, and does not necessarily reflect the current state. The current state is available on the following page: Brigham Inc., Boston, Morocco.
The visualizations for "Brigham Inc. - EU funding (€245,732): Dynamic regulation of paracellular channel gating"
are provided by
North Data
and may be reused under the terms of the
Creative Commons CC-BY license.
